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ief buffer concentrate  (Cytiva Europe)


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    Structured Review

    Cytiva Europe ief buffer concentrate
    Ief Buffer Concentrate, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 95/100, based on 3283 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ief+buffer/IPG+buffer/pm41742785-135-59-64
    Average 95 stars, based on 3283 article reviews
    ief buffer concentrate - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Electrofocusing:

    Article Title: Immobilized pH gradient-driven paper-based IEF: a new method for fractionating complex peptide mixtures before MS analysis
    Article Snippet: Notably, an IPGphor strip holder with cup loading (GE Healthcare) was used instead of regular strip holders, although no cup loading of the samples was employed. .. The IEF strip was rehydrated in a rehydration tray overnight at room temperature (RT) in 450 μl of an IEF buffer (7 M urea/2 M thiourea/1% DTT/1% 3-10 ampholytes) with gel face down and overlaid with 1.5 mL of dry strip cover fluid (GE Healthcare). ..

    Article Title: Human Mesenchymal Stem Cell Expression Program upon Extended Ex-Vivo Cultivation, as Revealed by 2-DE-Based Quantitative Proteomics
    Article Snippet: For the isoelectric focusing (IEF) of the protein samples, Immobiline DryStrips with 24 cm and a 3–10 non-linear pH range (GE Healthcare) were rehydrated overnight in 450 μL of reswelling buffer (7 M urea, 2 M thiourea, 2% (w/v) CHAPS, 0.002% (w/v) bromophenol blue, 1.2% (v/v) DeStreak (GE Healthcare) and 0.5% (v/v) pharmalytes (GE Healthcare)). .. Two replicates of each sample containing proteins from P3, P7 or from the internal standard samples were gently resuspended in 80 μL of IEF buffer (7 M urea, 2 M thiourea, 2% (w/v) CHAPS, 0.002% (w/v) bromophenol blue, 15 mM DTT and 2% (v/v) pharmalytes 3–10 (GE Healthcare)), prior to anodic cup loading on the Immobiline DryStrips. ..

    Article Title: Localization of trypsin-like protease in postmortem tissue of white shrimp (Litopenaeus vannamei) and its effect in muscle softening.
    Article Snippet: Fluorescein-isothiocyanate (FITC) labeled trypsin-like protease was prepared and injected into the hepatopancreas of white shrimp.. Different segments of the injected shrimp were analyzed with a fluorescence microscope during storage.. FITC-trypsin-like protease can be detected in the first segment of shrimp muscle at day 4, while it cannot be observed in the second segment until day 6.

    Article Title: Proteome changes in the skin of the grape cultivar Barbera among different stages of ripening
    Article Snippet: .. The final pellet was dried under vacuum and dissolved in IEF buffer [7 M urea, 2 M thiourea, 3% (w/v) CHAPS, 1% (v/v) NP-40, 50 mg mL -1 DTT and 2% (v/v) IPG Buffer pH 3–10 (GE Healthcare)] by vortexing and incubating for 1 h at room temperature. ..

    Article Title: Proteomic characterization of iron deficiency responses in Cucumis sativus L. roots
    Article Snippet: .. The final pellet was dried under vacuum and dissolved in IEF buffer [7 M urea, 2 M thiourea, 3% (w/v) CHAPS, 1% (v/v) NP-40, 50 mg mL -1 DTT and 2% (v/v) IPG Buffer pH 4-7 (GE Healthcare Life Sciences, USA)] by vortexing and incubating for 1 h at room temperature. ..

    Article Title: Evaluation of protein pattern changes in roots and leaves of Zea mays plants in response to nitrate availability by two-dimensional gel electrophoresis analysis
    Article Snippet: .. The final pellet was dried under vacuum and dissolved in IEF buffer [7 M urea, 2 M thiourea, 3% (w/v) CHAPS, 1% (v/v) octylphenoxy polyethoxy ethanol (NP-40), 50 mg mL -1 DTT and 2% (v/v) IPG Buffer pH 3–10 (GE Healthcare)] by vortexing and incubating for 1 h at room temperature. ..

    Article Title: Inhibition of MNK pathways enhances cancer cell response to chemotherapy with temozolomide and targeted radionuclide therapy.
    Article Snippet: Inhibition of MNK pathways enhances cancer cell response to chemotherapy with temozolomide and targeted radionuclide therapy Michal Grzmil, Jan Seebacher, Daniel Hess, Martin Behe, Roger Schibli, Gerald Moncayo, Stephan Frank, Brian A. Hemmings PII: S0898-6568(16)30139-5 DOI: doi: 10.1016/j.cellsig.2016.06.005 Reference: CLS 8706 To appear in: Cellular Signalling Received date: 12 May 2016 Revised date: 7 June 2016 Accepted date: 7 June 2016 Please cite this article as: Michal Grzmil, Jan Seebacher, Daniel Hess, Martin Behe, Roger Schibli, Gerald Moncayo, Stephan Frank, Brian A. Hemmings, Inhibition of MNK pathways enhances cancer cell response to chemotherapy with temozolomide and targeted radionuclide therapy, Cellular Signalling (2016), doi: 10.1016/j.cellsig.2016.06.005 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

    Article Title: The Predicted ABC Transporter AbcEDCBA Is Required for Type IV Secretion System Expression and Lysosomal Evasion by Brucella ovis
    Article Snippet: .. All labeled proteins (150 μg) were added to 3.4 μL of immobilized pH gradient (IPG) buffer (10 μL/mL), 450 μg of unlabeled protein, and IEF buffer (8 M urea, 2 M thiourea, 4% CHAPS, 0.0025% bromophenol blue, 10 mg/mL dithiothreitol) in a total volume of 340 μL per IPG strip (18 cm, pH 4–7, GE Healthcare). .. The samples were incubated overnight with the IPG strips, submitted to isoelectric focusing using Ettan IPGphor system (GE Healthcare), followed by incubation in equilibrium solution (50 mM Tris-HCl, pH 8.8, 6 M urea, 30% glycerol, 2% SDS, 0.002% bromophenol blue, and 125 mM DTT) for 15 min and an additional incubation in a new solution containing 13.5 mM iodoacetamide instead of DTT.

    Stripping Membranes:

    Article Title: Immobilized pH gradient-driven paper-based IEF: a new method for fractionating complex peptide mixtures before MS analysis
    Article Snippet: Notably, an IPGphor strip holder with cup loading (GE Healthcare) was used instead of regular strip holders, although no cup loading of the samples was employed. .. The IEF strip was rehydrated in a rehydration tray overnight at room temperature (RT) in 450 μl of an IEF buffer (7 M urea/2 M thiourea/1% DTT/1% 3-10 ampholytes) with gel face down and overlaid with 1.5 mL of dry strip cover fluid (GE Healthcare). ..

    Article Title: Inhibition of MNK pathways enhances cancer cell response to chemotherapy with temozolomide and targeted radionuclide therapy.
    Article Snippet: Inhibition of MNK pathways enhances cancer cell response to chemotherapy with temozolomide and targeted radionuclide therapy Michal Grzmil, Jan Seebacher, Daniel Hess, Martin Behe, Roger Schibli, Gerald Moncayo, Stephan Frank, Brian A. Hemmings PII: S0898-6568(16)30139-5 DOI: doi: 10.1016/j.cellsig.2016.06.005 Reference: CLS 8706 To appear in: Cellular Signalling Received date: 12 May 2016 Revised date: 7 June 2016 Accepted date: 7 June 2016 Please cite this article as: Michal Grzmil, Jan Seebacher, Daniel Hess, Martin Behe, Roger Schibli, Gerald Moncayo, Stephan Frank, Brian A. Hemmings, Inhibition of MNK pathways enhances cancer cell response to chemotherapy with temozolomide and targeted radionuclide therapy, Cellular Signalling (2016), doi: 10.1016/j.cellsig.2016.06.005 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

    Article Title: The Predicted ABC Transporter AbcEDCBA Is Required for Type IV Secretion System Expression and Lysosomal Evasion by Brucella ovis
    Article Snippet: .. All labeled proteins (150 μg) were added to 3.4 μL of immobilized pH gradient (IPG) buffer (10 μL/mL), 450 μg of unlabeled protein, and IEF buffer (8 M urea, 2 M thiourea, 4% CHAPS, 0.0025% bromophenol blue, 10 mg/mL dithiothreitol) in a total volume of 340 μL per IPG strip (18 cm, pH 4–7, GE Healthcare). .. The samples were incubated overnight with the IPG strips, submitted to isoelectric focusing using Ettan IPGphor system (GE Healthcare), followed by incubation in equilibrium solution (50 mM Tris-HCl, pH 8.8, 6 M urea, 30% glycerol, 2% SDS, 0.002% bromophenol blue, and 125 mM DTT) for 15 min and an additional incubation in a new solution containing 13.5 mM iodoacetamide instead of DTT.

    Labeling:

    Article Title: The Predicted ABC Transporter AbcEDCBA Is Required for Type IV Secretion System Expression and Lysosomal Evasion by Brucella ovis
    Article Snippet: .. All labeled proteins (150 μg) were added to 3.4 μL of immobilized pH gradient (IPG) buffer (10 μL/mL), 450 μg of unlabeled protein, and IEF buffer (8 M urea, 2 M thiourea, 4% CHAPS, 0.0025% bromophenol blue, 10 mg/mL dithiothreitol) in a total volume of 340 μL per IPG strip (18 cm, pH 4–7, GE Healthcare). .. The samples were incubated overnight with the IPG strips, submitted to isoelectric focusing using Ettan IPGphor system (GE Healthcare), followed by incubation in equilibrium solution (50 mM Tris-HCl, pH 8.8, 6 M urea, 30% glycerol, 2% SDS, 0.002% bromophenol blue, and 125 mM DTT) for 15 min and an additional incubation in a new solution containing 13.5 mM iodoacetamide instead of DTT.



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    Image Search Results


    Isoelectic focusing (IEF) of the bee venom fractions 1–7 in Ready Gel Precast Gels with ampholytes of pH gradient from 3.0 to 10.5. A multi-sample horizontal IEF gel was employed with 11 lanes containing the seven bee venom fractions and four unrelated samples. For presentation in , the lanes of the seven bee venom fractions were excised from the original IEF gel photograph and are arranged adjacently to facilitate comparative analysis. Lane 1 includes tube 3–6, lane 2 includes tubes 7–9, lane 3 includes tubes 10–11, lane 4 includes tubes 12–13, lane 5 includes tubes 14–16, lane 6 includes tubes 27–30, and lane 7 includes tubes 31–36, as shown in . The standard p I markers (FMC Corporation, Rockland, ME, USA) sample of a p I range from 4.65 to 10.6 is applied on the unmarked lane on the far left.

    Journal: Pharmaceuticals

    Article Title: Bee Venom Proteins Enhance Proton Absorption by Membranes Composed of Phospholipids of the Myelin Sheath and Endoplasmic Reticulum: Pharmacological Relevance

    doi: 10.3390/ph18091334

    Figure Lengend Snippet: Isoelectic focusing (IEF) of the bee venom fractions 1–7 in Ready Gel Precast Gels with ampholytes of pH gradient from 3.0 to 10.5. A multi-sample horizontal IEF gel was employed with 11 lanes containing the seven bee venom fractions and four unrelated samples. For presentation in , the lanes of the seven bee venom fractions were excised from the original IEF gel photograph and are arranged adjacently to facilitate comparative analysis. Lane 1 includes tube 3–6, lane 2 includes tubes 7–9, lane 3 includes tubes 10–11, lane 4 includes tubes 12–13, lane 5 includes tubes 14–16, lane 6 includes tubes 27–30, and lane 7 includes tubes 31–36, as shown in . The standard p I markers (FMC Corporation, Rockland, ME, USA) sample of a p I range from 4.65 to 10.6 is applied on the unmarked lane on the far left.

    Article Snippet: The following materials and chemicals were used in this study: Phospholipids—phosphatidylserine, sphingomyelin, phosphatidylinositol, phosphatidylcholine, and phosphatidylethanolamine—were purified from the rat liver (see the Preparations section below), Dichloro-diphenyl-trichloroethane (DDT), Sephadex G-25 and CM Sephadex C-50 (Nanjing Duly Biotech Co., Ltd., Nanjing, China), Tris(hydroxymethyl)aminomethane (Tris) 10 M pH 8.5 buffer, 1.0 M Tris-HCl pH 6.8 with 0.4% SDS buffer, Bromo-phenol Blue (Thomas Scientific, Swedesboro, NJ, USA); Mini-PROTEAN TGX precast gels (8% density), Isoelectric Focusing Gel Sample Buffer (IEF Gel), Ready Gel Precast Gels with ampholytes making pH gradient 3–10.5, 10× IEF Anode Buffer, 10× IEF Cathode Buffer (Bio-Rad Laboratories Co., Ltd., Shanghai, China), IEF p I 4.65–10.6 range protein markers for IEF (Shanghai Yeyuan Biotechnology Co., Ltd., Shanghai, China), Sodium Dodecyl Sulfate (SDS), 50× TAE (Tris-acetate-EDTA, pH 8.3) buffer, Coomassie Brilliant Blue-R-250, low-molecular-weight markers for SDS-PAGE (Thermo Fisher Scientific Inc., Shanghai, China), lyophilized bee venom (Sigma Aldrich, Saint Louis, MO, USA), 3.5 kDa cutoff dialysis tubing (Sigma Aldrich, Saint Louis, MO, USA), research grade Glycine (Asiamerica Group, Inc., Westwood, NJ, USA); Deionized-Distilled water (dd-H 2 O) (XiZhiMeng Co., Ltd., Shanghai, China).

    Techniques:

    Dot blot of B. papyrifera pollen-protein extract. Samples of 1 µL of pure pollen-protein extracts and 1/10 diluted pollen-protein extracts in 1X PBS blotted on a nitrocellulose paper against 1:1000 diluted anti-IgE monoclonal HRP Southern Biotech antibodies. The dark spots show IgE binding with the respective serum ID given above the spot.

    Journal: Metabolites

    Article Title: Broussonetia papyrifera Pollen Metabolome Insights, Allergenicity, and Dispersal in Response to Climate Change Variables

    doi: 10.3390/metabo15020137

    Figure Lengend Snippet: Dot blot of B. papyrifera pollen-protein extract. Samples of 1 µL of pure pollen-protein extracts and 1/10 diluted pollen-protein extracts in 1X PBS blotted on a nitrocellulose paper against 1:1000 diluted anti-IgE monoclonal HRP Southern Biotech antibodies. The dark spots show IgE binding with the respective serum ID given above the spot.

    Article Snippet: Water-soluble pollen proteins were extracted in Bio-Rad TM 1X PBS buffer Cat # 1610763.

    Techniques: Dot Blot, Binding Assay